How can variable handling differ when including files with require_once in PHP?

When including files with require_once in PHP, variable handling can differ due to the scope in which the variables are defined. To ensure that variables are accessible in the included file, they should be defined before including the file. This can be done by passing the variables as arguments to functions or using global variables.

// Define variables before including the file
$variable = "Hello";

// Include the file with require_once
require_once 'included_file.php';
```

In the included_file.php:

```php
// Access the variable defined in the main file
echo $variable;