Is it possible to manipulate a session file by repeatedly accessing it, and how can this be prevented?

It is possible to manipulate a session file by repeatedly accessing it if proper security measures are not in place. To prevent this, you can regenerate the session ID periodically to limit the window of opportunity for attackers to access and manipulate the session file. 

// Regenerate session ID every 30 minutes
if (isset($_SESSION['last_regenerated']) && time() - $_SESSION['last_regenerated'] > 1800) {
    session_regenerate_id(true);
    $_SESSION['last_regenerated'] = time();
}

Keywords

PHP session file manipulation security prevention

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