How does the session_start() function impact the availability of $_SESSION variables in PHP scripts?

The session_start() function is essential for initializing a session in PHP, which allows the server to store session variables like $_SESSION. Without calling session_start(), the $_SESSION variables will not be available in the script. To ensure the availability of $_SESSION variables, always include session_start() at the beginning of your PHP scripts.

<?php
session_start();

// Now you can use $_SESSION variables in this script
$_SESSION['username'] = 'JohnDoe';
?>