How does the session_start() function impact session variable handling in PHP?

The session_start() function is used to start a new or resume an existing session in PHP. It must be called before any output is sent to the browser to ensure proper session variable handling. Without session_start(), session variables cannot be accessed or set in the PHP script.

<?php
session_start();

// Now you can work with session variables
$_SESSION['username'] = 'JohnDoe';
?>