How can the output of a PHP file be affected when included using the PHP include function compared to JavaScript?

When including a PHP file using the `include` function, the output of the included file will be directly embedded into the main file, affecting the final output. On the other hand, when including a JavaScript file, the code will be executed by the browser separately, without directly affecting the PHP output. To prevent PHP file output from affecting the main file, you can use output buffering to capture the included file's output and then manipulate it as needed.

<?php
ob_start();
include 'included_file.php';
$output = ob_get_clean();
// Manipulate $output as needed
echo $output;
?>