How can the HTML source code of a PHP file affect the output of variables and functions?

The HTML source code of a PHP file can affect the output of variables and functions if there are syntax errors or improper placement of PHP tags within the HTML code. To solve this issue, ensure that PHP code is enclosed within <?php ?> tags and placed in appropriate locations within the HTML code.

&lt;!DOCTYPE html&gt;
&lt;html&gt;
&lt;head&gt;
    &lt;title&gt;PHP Output&lt;/title&gt;
&lt;/head&gt;
&lt;body&gt;
    &lt;h1&gt;&lt;?php echo &quot;Hello, World!&quot;; ?&gt;&lt;/h1&gt;
&lt;/body&gt;
&lt;/html&gt;