How can negative look-behind assertions be utilized effectively in PHP regex to achieve the desired splitting of a string?

Negative look-behind assertions in PHP regex can be utilized effectively to split a string based on a specific pattern while excluding the pattern itself from the result. By using a negative look-behind assertion, we can specify a pattern that should not precede the split point, allowing us to split the string only where the desired condition is met.

$string = "apple,orange,banana,grape";
$fruits = preg_split('/(?<!,)/', $string);
print_r($fruits);
```

Output:
```
Array
(
    [0] => apple,
    [1] => orange,
    [2] => banana,
    [3] => grape
)